Identification and enrichment of anaerobic digestion consortia is continued towards the construction of an advanced composite bioaugmentation inocula. Batch acclimatisation experiments are being continued at the lab to further develop hydrolytic and methanogenic consortia as well as the . Ammonia-tolerant methanogens were successfully acclimatised and enriched (≥5 g/L NH4+-N) earlier in the project. Targeted strategies were implemented and different growth parameters (i.e., methane production, VFA levels, pH, maximum growth rate, etc.) were evaluated towards further identification of the performance and robustness of the final enriched cultures under high lignocellulose or ammonia conditions. .
The ammonia tolerant methanogenic consortia with Methanoculleus bourgensis (M. bourgensis) and the bacteria Ruminococcus Albus, which displayed the most promising results were used in the advanced composite biougmentation inocula. Furthermore, the inoculum, that was created as a composite formulation, undergoes further preservation based on the optimal strategy identified in WP3. The implementation of lyophilisation experiments with the developed optimum lyophilisation method for the consortia was performed and further results are pending.